This book contains all the necessary information and advice for anyone wishing to obtain electron micrographs showing the most accurate ultrastructural detail in thin sections of any type of biological specimen.
The guidelines for the choice of preparative methods are based on an extensive survey of current laboratory practice. For the first time, in a textbook of this kind, the molecular events occurring during fixation and embedding are analysed in detail. The reasons for choosing particular specimen preparation methods are explained and guidance is given on how to modify established techniques to suit individual requirements.
All the practical methods advocated are clearly described, with accompanying tables and the results obtainable are illustrated with many electron micrographs.
Portland Press Series: Practical Methods in Electron Microscopy, Volume 17, Audrey M. Glauert, Editor
Originally published in 1999.
The Princeton Legacy Library uses the latest print-on-demand technology to again make available previously out-of-print books from the distinguished backlist of Princeton University Press. These editions preserve the original texts of these important books while presenting them in durable paperback and hardcover editions. The goal of the Princeton Legacy Library is to vastly increase access to the rich scholarly heritage found in the thousands of books published by Princeton University Press since its founding in 1905.
"Since 1972, when the first volume of Practical Methods in Electron Microscopy appeared under the critical eye and constructive editorial guidance of Audrey Glauert, users of the microscope have eagerly snapped up each new title. The 17th volume in the series has now appeared, Biological Specimen Preparation for Transmission Electron Microscopy by Audrey Glauert herself together with Peter R Lewis, and the high standards of lucidity and precision that Audrey Glauert has imposed on her authors are more than maintained here. The ten chapters cover the obvious themes: fixation, embedding and safety... Beautifully printed, clearly and knowledgeably written, this is a model of what we have come to expect from the 'Practical Methods' series. I look forward to a future in which authors, editor and publisher continue to contribute to the 'Science and Art of Electron Microscopy', concludes Audrey Glauert in her editorial preface - and so do we."--Peter Hawkes, Ultramicroscopy
Table of Contents:
- An introduction to fixation and embedding procedures and their safe use in the laboratory
- Fixation methods
- Dehydration methods
- Embedding methods
- Embedding in epoxy resins
- Embedding in acrylic resins
- The Lowicryl resins and embedding at low temperatures
- Other embedding media
- Processing schedules
- Appendix: List of suppliers
- Subject Index
Hardcover: For sale only in the United States, Canada, Central and South America, and the Caribbean Islands
Paperback: For sale only in the United States, Canada, Central and South America, and the Caribbean Islands