- Biological Specimen Preparation for Transmission Electron Microscopy
Biological Specimen Preparation for Transmission Electron Microscopy
Audrey M. Glauert & Peter R. Lewis
- Paperback2014Paperback62.000.00ISBN9780691600154350 pp.7 x 10Live
- Hardcover2016Hardcover155.00129.95ISBN9780691630120350 pp.7 x 10Live
This book contains all the necessary information and advice for anyone wishing to obtain electron micrographs showing the most accurate ultrastructural detail in thin sections of any type of biological specimen.
The guidelines for the choice of preparative methods are based on an extensive survey of current laboratory practice. For the first time, in a textbook of this kind, the molecular events occurring during fixation and embedding are analysed in detail. The reasons for choosing particular specimen preparation methods are explained and guidance is given on how to modify established techniques to suit individual requirements.
All the practical methods advocated are clearly described, with accompanying tables and the results obtainable are illustrated with many electron micrographs.
Portland Press Series: Practical Methods in Electron Microscopy, Volume 17, Audrey M. Glauert, Editor
First published in 1999.
The Princeton Legacy Library uses the latest print-on-demand technology to again make available previously out-of-print books from the distinguished backlist of Princeton University Press. These editions preserve the original texts of these important books while presenting them in durable paperback and hardcover editions. The goal of the Princeton Legacy Library is to vastly increase access to the rich scholarly heritage found in the thousands of books published by Princeton University Press since its founding in 1905.
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"The two chapters dealing with embedding cover a wide range of both media and techniques. The inclusion of topics such as stability of the polymerized resin in the electron microscope and of advanced techniques such as vacuum infiltration make this a thorough review of this area... New chapters which focus on cytochemistry and cryofixation are invaluable resources especially for those just starting out in these areas. The chapter that deals specifically with low temperature embedding is very timely... The culmination of this book with a chapter giving specific processing schedules is very useful... Overall this is an excellent book and keeps to the high standards Audrey Glauert has set with her past volumes."--Richard Cole, Microscopy and Analysis
"Electron microscopy has been through something of a decline in recent years. The high cost of buying and maintaining electron microscopes, the lack of skilled expertise and competition from confocal microscopes have all combined to make these research tools slightly unfashionable. It is a shame and in my opinion short-sighted. It is all very well for biochemists to discover new proteins and molecular biologists to engineer new genes but what is actually happening in the cells? Where are these proteins? What effect is this new gene having? Often the best clue comes from electron microscopy, with it's superior resolving power: the books in this series are a valuable fund of information that allow us to look inside cells and help us visualize all manner of cellular processes.
"So, who is this book directed at? Certainly, it is probably too specialized for undergraduates and it won't give the general reader a basic grounding in electron microscopy. Rather, as is the case with the others in the series, this is an excellent reference book and probably the first place I would turn to for information on specific applications. It is all very well consulting specific references for individual protocols but in my experience they often don't run as smoothly as I would hope. By contrast, the methods outlined here have been well tried and tested and consequently have a much greater chance of success. Does this, I wonder, make Audrey Glauert the Delia Smith of electron microscopy?"--Anton Page, The Biochemist
Table of Contents
- An introduction to fixation and embedding procedures and their safe use in the laboratory
- Fixation methods
- Dehydration methods
- Embedding methods
- Embedding in epoxy resins
- Embedding in acrylic resins
- The Lowicryl resins and embedding at low temperatures
- Other embedding media
- Processing schedules
- Appendix: List of suppliers
- Subject Index
Other Books Written by this Author(s)
- Audrey M. Glauert
- Peter R. Lewis
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